OPP say the man got out of his car and was on foot when he was struck by a tractor trailer just before 4 a.m. Saturday.
However, the investigation is ongoing and police are asking anyone who was in the area at the time to contact them.
The westbound lanes of Highway 401 were detoured at Joyceville Road, and police expected to have them reopened by noon.
Fronteanc OPP says they were called to Highway 401, about 1 kilometre east of Highway 15 at 3:50 a.m. for a report of a pedestrian struck.
CLEARED:ROAD CLOSURE: #Hwy401 WB at Joyceville Rd #Kingston– Highway is now OPEN. ^ag pic.twitter.com/B0s7t8Ay62
A pedestrian was struck and killed by a passing tractor trailer on Hwy. 401 near Kingston early Saturday, Ontario Provincial Police reported.
The male victim was pronounced dead at the scene. The driver of the tractor trailer remained at the scene.
OPP officers from the Frontenac detachment responded to the incident just before 4 a.m. The unidentified man was pronounced dead at the scene.
OPP Technical Collision Investigators were examining the scene on Saturday. Westbound lanes of Hwy. 401 at Joyceville Road reopened just before 1:30 p.m.
The OPP requested that anyone who may have been in the area at the time of the collision or might have information about it contact the Frontenac OPP at 1-888-310-1122.